FineTest
SKU:EH4333
Human HD3(Histone Deacetylase 3) ELISA Kit
Human HD3(Histone Deacetylase 3) ELISA Kit
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Product Name
Human HD3(Histone Deacetylase 3) ELISA Kit
Alias
RPD3-2 ELISA Kit, SMAP45 ELISA Kit, HDAC3 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
HD3 ELISA Kit allows for the in vitro quantitative determination of HD3 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of HD3 and the recovery rates were calculated by comparing the measured value to the expected amount of HD3 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
87-103 |
94 |
|
EDTA plasma(n=5) |
85-95 |
93 |
|
heparin plasma(n=5) |
90-99 |
94 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HD3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
87-104% |
87-102% |
86-105% |
|
EDTA plasma(n=5) |
83-100% |
88-94% |
85-101% |
|
heparin plasma(n=5) |
81-98% |
82-98% |
88-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
