FineTest
SKU:EH4326
Human a1AGP(total alpha 1-acid glycoprotein) ELISA Kit
Human a1AGP(total alpha 1-acid glycoprotein) ELISA Kit
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Product Name
Human a1AGP(total alpha 1-acid glycoprotein) ELISA Kit
Alias
Alpha-1-Acid Glycoprotein ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
a1AGP ELISA Kit allows for the in vitro quantitative determination of a1AGP concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
4.688-300ng/ml
Sensitivity
< 2.813ng/ml
Species
Human
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of a1AGP and the recovery rates were calculated by comparing the measured value to the expected amount of a1AGP in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
90-101 |
96 |
|
EDTA plasma(n=5) |
92-101 |
96 |
|
heparin plasma(n=5) |
85-105 |
93 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of a1AGP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
88-104% |
85-103% |
94-104% |
|
EDTA plasma(n=5) |
83-99% |
84-97% |
84-97% |
|
heparin plasma(n=5) |
92-100% |
83-94% |
84-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
