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SKU:EH13894

Human BCKDHA (2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial) ELISA Kit

Human BCKDHA (2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial) ELISA Kit

Regular price $450.00 USD
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size
Sensitivity
Range

Product Name

Human BCKDHA (2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial) ELISA Kit

Alias

BCKDHA ELISA Kit2-oxoisovalerate dehydrogenase subunit alpha ELISA Kit mitochondrial ELISA KitBranched-chain alpha-keto acid dehydrogenase E1 component alpha chain ELISA KitBCKDE1A ELISA KitBCKDH E1-alpha ELISA Kit

Detection Method

Sandwich ELISA, Double Antibody

Application

 BCKDHA  ELISA Kit allows for the in vitro quantitative determination of BCKDHA concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.313-20ng/ml

Sensitivity

< 0.188ng/ml

Species

Human

UniProt ID

P12694

Standard Curve

Storage

2-8 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of BCKDHA and the recovery rates were calculated by comparing the measured value to the expected amount of BCKDHA in samples.

Matrix

Recovery range(%)

Average(%)

serum(n=5)

85-104

94

EDTA plasma(n=5)

90-104

97

heparin plasma(n=5)

85-102

95

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BCKDHA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

serum(n=5)

86-96%

86-95%

86-104%

EDTA plasma(n=5)

85-101%

91-101%

86-97%

heparin plasma(n=5)

81-100%

85-100%

88-100%

CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

 

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