FineTest
SKU:EH12120
Human SCUBE3(Signal peptide, CUB and EGF-like domain-containing protein 3) ELISA Kit
Human SCUBE3(Signal peptide, CUB and EGF-like domain-containing protein 3) ELISA Kit
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Product Name
Human SCUBE3(Signal peptide, CUB and EGF-like domain-containing protein 3) ELISA Kit
Alias
CEGF3 ELISA Kit, SCUBE3 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
SCUBE3 ELISA Kit allows for the in vitro quantitative determination of SCUBE3 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of SCUBE3 and the recovery rates were calculated by comparing the measured value to the expected amount of SCUBE3 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
86-105 |
95 |
|
EDTA plasma(n=5) |
87-99 |
93 |
|
heparin plasma(n=5) |
88-102 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SCUBE3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
87-103% |
91-103% |
89-101% |
|
EDTA plasma(n=5) |
86-99% |
83-99% |
84-97% |
|
heparin plasma(n=5) |
82-96% |
81-92% |
84-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
