FineTest
SKU:EB0081
Bovine E2(Estradiol) ELISA Kit
Bovine E2(Estradiol) ELISA Kit
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Product Name
Bovine E2(Estradiol) ELISA Kit
Alias
E2 ELISA Kit, Estradiol ELISA Kit
Detection Method
Competitive ELISA, Coated with Antigen
Application
E2 ELISA Kit allows for the in vitro quantitative determination of E2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
12.5-800pg/ml
Sensitivity
< 7.5pg/ml
Species
Bovine
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of E2 and the recovery rates were calculated by comparing the measured value to the expected amount of E2 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
87-104 |
94 |
|
EDTA plasma(n=5) |
89-98 |
91 |
|
heparin plasma(n=5) |
88-102 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of E2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
87-98% |
88-99% |
86-95% |
|
EDTA plasma(n=5) |
82-93% |
83-101% |
93-100% |
|
heparin plasma(n=5) |
80-93% |
82-92% |
80-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
