FineTest
SKU:EB0043
Bovine CD86(cluster of differentiation 86) ELISA Kit
Bovine CD86(cluster of differentiation 86) ELISA Kit
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Product Name
Estrone(Estrone) ELISA Kit
Alias
Estrone ELISA Kit
Detection Method
Competitive ELISA, Coated with Antigen
Application
Estrone ELISA Kit allows for the in vitro quantitative determination of Estrone concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10pmol/ml
Sensitivity
< 0.094pmol/ml
Species
Universal
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Estrone and the recovery rates were calculated by comparing the measured value to the expected amount of Estrone in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
86-104 |
95 |
|
EDTA plasma(n=5) |
87-101 |
95 |
|
heparin plasma(n=5) |
90-104 |
99 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Estrone and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
87-105% |
91-102% |
90-105% |
|
EDTA plasma(n=5) |
89-101% |
84-96% |
83-101% |
|
heparin plasma(n=5) |
84-99% |
81-92% |
80-93% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
