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SKU:EU2582
5-HIAA(5-Hydroxyindoleacetic Acid) ELISA Kit
5-HIAA(5-Hydroxyindoleacetic Acid) ELISA Kit
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Product Name
5-HIAA(5-Hydroxyindoleacetic Acid) ELISA Kit
Alias
5-HIAA ELISA Kit, 5-Hydroxyindoleacetic Acid ELISA Kit
Detection Method
Competitive ELISA, Coated with Antigen
Application
5-HIAA ELISA Kit allows for the in vitro quantitative determination of 5-HIAA concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
1.563-100ng/ml
Sensitivity
< 0.938ng/ml
Species
Universal
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of 5-HIAA and the recovery rates were calculated by comparing the measured value to the expected amount of 5-HIAA in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
88-100 |
95 |
|
EDTA plasma(n=5) |
85-98 |
93 |
|
heparin plasma(n=5) |
86-104 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 5-HIAA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
89-103% |
87-102% |
90-95% |
|
EDTA plasma(n=5) |
84-100% |
85-98% |
82-101% |
|
heparin plasma(n=5) |
81-98% |
83-93% |
87-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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