FineTest
SKU:EU2560
3-NT(3-Nitrotyrosine) ELISA Kit
3-NT(3-Nitrotyrosine) ELISA Kit
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Product Name
3-NT(3-Nitrotyrosine) ELISA Kit
Alias
3-NT ELISA Kit, 3-Nitrotyrosine ELISA Kit
Detection Method
Competitive ELISA, Coated with Antigen
Application
3-NT ELISA Kit allows for the in vitro quantitative determination of 3-NT concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
1.563-100ng/ml
Sensitivity
< 0.938ng/ml
Species
Universal
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of 3-NT and the recovery rates were calculated by comparing the measured value to the expected amount of 3-NT in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
91-103 |
99 |
|
EDTA plasma(n=5) |
85-105 |
97 |
|
heparin plasma(n=5) |
89-101 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 3-NT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-105% |
93-105% |
93-105% |
|
EDTA plasma(n=5) |
88-101% |
82-100% |
93-101% |
|
heparin plasma(n=5) |
81-94% |
81-100% |
87-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
