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SKU:EU2561
8-epi-PGF2α(8-Epi-Prostaglandin F2 Alpha) ELISA Kit
8-epi-PGF2α(8-Epi-Prostaglandin F2 Alpha) ELISA Kit
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Product Name
8-iso-PGF2α(8-isoprostane) ELISA Kit
Alias
8-epi-PGF2α ELISA Kit, 8-Epi-Prostaglandin F2 Alpha ELISA Kit, 8-iso-PGF2α ELISA Kit
Detection Method
Competitive ELISA, Coated with Antigen
Application
8-iso-PGF2α ELISA Kit allows for the in vitro quantitative determination of 8-iso-PGF2α concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Universal
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of 8-iso-PGF2α and the recovery rates were calculated by comparing the measured value to the expected amount of 8-iso-PGF2α in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
87-101 |
95 |
|
EDTA plasma(n=5) |
85-97 |
93 |
|
heparin plasma(n=5) |
88-104 |
98 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 8-iso-PGF2α and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
87-103% |
87-95% |
92-102% |
|
EDTA plasma(n=5) |
82-99% |
82-98% |
85-98% |
|
heparin plasma(n=5) |
87-96% |
81-97% |
80-93% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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