FineTest
SKU:EU0461
17-OHP(17-Hydroxyprogesterone) ELISA Kit
17-OHP(17-Hydroxyprogesterone) ELISA Kit
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Product Name
17-OHP(17-Hydroxyprogesterone) ELISA Kit
Alias
17-OHP ELISA Kit, 17-Hydroxyprogesterone ELISA Kit
Detection Method
Competitive ELISA, Coated with Antigen
Application
17-OHP ELISA Kit allows for the in vitro quantitative determination of 17-OHP concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
1.563-100pg/ml
Sensitivity
< 0.938pg/ml
Species
Universal
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of 17-OHP and the recovery rates were calculated by comparing the measured value to the expected amount of 17-OHP in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
89-102 |
94 |
|
EDTA plasma(n=5) |
88-101 |
97 |
|
heparin plasma(n=5) |
92-104 |
97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 17-OHP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-102% |
85-95% |
87-104% |
|
EDTA plasma(n=5) |
90-100% |
84-101% |
85-95% |
|
heparin plasma(n=5) |
87-93% |
81-94% |
82-96% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
