FineTest
SKU:EU0251
2-ME2(2-Methoxy Estradiol) ELISA Kit
2-ME2(2-Methoxy Estradiol) ELISA Kit
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Product Name
2-ME2(2-Methoxy Estradiol) ELISA Kit
Alias
2-ME2 ELISA Kit
Detection Method
Competitive ELISA, Coated with Antigen
Application
2-ME2 ELISA Kit allows for the in vitro quantitative determination of 2-ME2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000ng/ml
Sensitivity
< 18.75ng/ml
Species
Universal
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of 2-ME2 and the recovery rates were calculated by comparing the measured value to the expected amount of 2-ME2 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
92-100 |
96 |
|
EDTA plasma(n=5) |
92-102 |
97 |
|
heparin plasma(n=5) |
86-102 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 2-ME2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-101% |
88-104% |
89-104% |
|
EDTA plasma(n=5) |
89-100% |
83-101% |
92-98% |
|
heparin plasma(n=5) |
88-100% |
80-99% |
88-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
