FineTest
SKU:ESH0010
Sheep IL-12(Interleukin 12) ELISA Kit
Sheep IL-12(Interleukin 12) ELISA Kit
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Product Name
Sheep IL-12(Interleukin 12) ELISA Kit
Alias
Interleukin 12 p70 ELISA Kit, CLMF p35 ELISA Kit, Cytotoxic lymphocyte matuRation factor 35 kDa subunit ELISA Kit, IL-12 subunit p35 ELISA Kit, IL-12 ELISA Kit, subunit p35 ELISA Kit, interleukin 12 ELISA Kit, p35 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
IL-12 ELISA Kit allows for the in vitro quantitative determination of IL-12 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 18.75pg/ml
Species
Sheep
UniProt ID
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IL-12 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-12 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
89-105 |
97 |
|
EDTA plasma(n=5) |
87-102 |
94 |
|
heparin plasma(n=5) |
90-105 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-12 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
91-97% |
89-105% |
85-102% |
|
EDTA plasma(n=5) |
86-96% |
82-101% |
85-100% |
|
heparin plasma(n=5) |
86-99% |
81-93% |
82-95% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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