FineTest
SKU:ESH0004
Sheep C3a(Complement Component 3a) ELISA Kit
Sheep C3a(Complement Component 3a) ELISA Kit
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Product Name
Sheep C3a(Complement Component 3a) ELISA Kit
Alias
C3a ELISA Kit, C3a-desArg ELISA Kit, ASP ELISA Kit, Anaphylatoxin ELISA Kit, C3 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
C3a ELISA Kit allows for the in vitro quantitative determination of C3a concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Sheep
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of C3a and the recovery rates were calculated by comparing the measured value to the expected amount of C3a in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
87-102 |
96 |
|
EDTA plasma(n=5) |
85-93 |
91 |
|
heparin plasma(n=5) |
95-104 |
99 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C3a and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
87-100% |
86-104% |
89-105% |
|
EDTA plasma(n=5) |
83-98% |
82-97% |
83-97% |
|
heparin plasma(n=5) |
81-93% |
83-92% |
82-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
