FineTest
SKU:ERB0091
Rabbit PAF(Platelet Activating Factor) ELISA Kit
Rabbit PAF(Platelet Activating Factor) ELISA Kit
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Product Name
Rabbit PAF(Platelet Activating Factor) ELISA Kit
Alias
PAF ELISA Kit, Platelet Activating Factor ELISA Kit
Detection Method
Competitive ELISA, Coated with Antigen
Application
PAF ELISA Kit allows for the in vitro quantitative determination of PAF concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Rabbit
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of PAF and the recovery rates were calculated by comparing the measured value to the expected amount of PAF in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-102 |
92 |
|
EDTA plasma(n=5) |
88-105 |
95 |
|
heparin plasma(n=5) |
92-101 |
96 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PAF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-102% |
85-105% |
85-102% |
|
EDTA plasma(n=5) |
82-98% |
83-94% |
82-101% |
|
heparin plasma(n=5) |
80-91% |
82-99% |
83-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
