FineTest
SKU:ERB0051
Rabbit IFN-γ(Interferon Gamma) ELISA Kit
Rabbit IFN-γ(Interferon Gamma) ELISA Kit
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Product Name
Rabbit IFN-γ(Interferon Gamma) ELISA Kit
Alias
IFN-γ ELISA Kit, IFNG ELISA Kit, IFG ELISA Kit, IFI ELISA Kit, Type II Interferon ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
IFN-γ ELISA Kit allows for the in vitro quantitative determination of IFN-γ concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
3.125-200pg/ml
Sensitivity
< 1.875pg/ml
Species
Rabbit
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IFN-γ and the recovery rates were calculated by comparing the measured value to the expected amount of IFN-γ in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
89-103 |
94 |
|
EDTA plasma(n=5) |
89-98 |
95 |
|
heparin plasma(n=5) |
86-105 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IFN-γ and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-104% |
85-102% |
86-95% |
|
EDTA plasma(n=5) |
85-97% |
83-98% |
95-100% |
|
heparin plasma(n=5) |
84-99% |
85-100% |
85-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
