FineTest
SKU:ERB0041
Rabbit ET-1(Endothelin 1) ELISA Kit
Rabbit ET-1(Endothelin 1) ELISA Kit
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Product Name
Rabbit ET-1(Endothelin 1) ELISA Kit
Alias
ET-1 ELISA Kit, EDN1 ELISA Kit, ET1 ELISA Kit, HDLCQ7 ELISA Kit, PPET1 ELISA Kit, Preproendothelin-1 ELISA Kit, endothelin 1 ELISA Kit, preproendothelin-1 ELISA Kit
Detection Method
Competitive ELISA, Coated with Antigen
Application
ET-1 ELISA Kit allows for the in vitro quantitative determination of ET-1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
1.25-80pg/ml
Sensitivity
< 0.625pg/ml
Species
Rabbit
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of ET-1 and the recovery rates were calculated by comparing the measured value to the expected amount of ET-1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
93-102 |
97 |
|
EDTA plasma(n=5) |
98-103 |
100 |
|
heparin plasma(n=5) |
89-103 |
97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ET-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-105% |
85-104% |
87-103% |
|
EDTA plasma(n=5) |
82-96% |
88-96% |
93-100% |
|
heparin plasma(n=5) |
82-98% |
85-99% |
89-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
