FineTest
SKU:ER1510
Rat GAGs(Glycosaminoglycan) ELISA Kit
Rat GAGs(Glycosaminoglycan) ELISA Kit
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Product Name
Rat GAGs(Glycosaminoglycan) ELISA Kit
Alias
Mucopolysaccharide ELISA Kit
Detection Method
Competitive ELISA, Coated with Antigen
Application
GAGs ELISA Kit allows for the in vitro quantitative determination of GAGs concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
1.563-100ng/ml
Sensitivity
< 0.938ng/ml
Species
Rat
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of GAGs and the recovery rates were calculated by comparing the measured value to the expected amount of GAGs in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
87-104 |
96 |
|
EDTA plasma(n=5) |
85-99 |
92 |
|
heparin plasma(n=5) |
86-98 |
94 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GAGs and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-105% |
86-105% |
85-103% |
|
EDTA plasma(n=5) |
82-100% |
87-100% |
84-95% |
|
heparin plasma(n=5) |
80-95% |
83-99% |
85-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
