FineTest
SKU:ER1462
Rat T(Testosterone) ELISA Kit
Rat T(Testosterone) ELISA Kit
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Product Name
Rat T(Testosterone) ELISA Kit
Alias
Testosterone ELISA Kit
Detection Method
Competitive ELISA, Coated with Antigen
Application
T ELISA Kit allows for the in vitro quantitative determination of T concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Rat
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of T and the recovery rates were calculated by comparing the measured value to the expected amount of T in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
86-104 |
95 |
|
EDTA plasma(n=5) |
86-104 |
93 |
|
heparin plasma(n=5) |
88-104 |
97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of T and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
89-99% |
87-105% |
86-88% |
|
EDTA plasma(n=5) |
89-98% |
82-100% |
89-101% |
|
heparin plasma(n=5) |
80-94% |
82-99% |
80-90% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
