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SKU:ER1464
Rat Histone H3(Hist2h3c2) ELISA Kit
Rat Histone H3(Hist2h3c2) ELISA Kit
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Product Name
Rat Histone H3(Hist2h3c2) ELISA Kit
Alias
H3 ELISA Kit, n ELISA Kit, H3 ELISA Kit, o ELISA Kit, H3F2 ELISA Kit, H3FM ELISA Kit, HIST2H3A ELISA Kit, HIST2H3C ELISA Kit, HIST2H3D ELISA Kit, histone cluster 2 ELISA Kit, H3a ELISA Kit, Histone H3 ELISA Kit, Histone H3.2 ELISA Kit, Histone H3 ELISA Kit, m ELISA Kit, Histone H3 ELISA Kit, o ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Histone H3 ELISA Kit allows for the in vitro quantitative determination of Histone H3 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Histone H3 and the recovery rates were calculated by comparing the measured value to the expected amount of Histone H3 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
88-101 |
96 |
|
EDTA plasma(n=5) |
88-103 |
98 |
|
heparin plasma(n=5) |
87-101 |
94 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Histone H3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-102% |
88-102% |
88-94% |
|
EDTA plasma(n=5) |
82-92% |
83-91% |
83-98% |
|
heparin plasma(n=5) |
81-94% |
80-100% |
81-91% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
