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SKU:ER1463

Rat 5-hydroxytryptamine ELISA Kit

Rat 5-hydroxytryptamine ELISA Kit

Regular price $400.00 USD
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size
Sensitivity
Range

Product Name

Rat 5-hydroxytryptamine ELISA Kit

Alias

5-hydroxytryptamine ELISA Kit

Detection Method

Competitive ELISA, Coated with Antigen

Application

5-hydroxytryptamine ELISA Kit allows for the in vitro quantitative determination of 5-hydroxytryptamine concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size

96T

Range

1.563-100ng/ml

Sensitivity

< 0.938ng/ml

Species

Rat

Standard Curve

Storage

2-8 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of 5-hydroxytryptamine and the recovery rates were calculated by comparing the measured value to the expected amount of 5-hydroxytryptamine in samples.

Matrix

Recovery range(%)

Average(%)

serum(n=5)

88-104

97

EDTA plasma(n=5)

91-103

96

heparin plasma(n=5)

87-98

93

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 5-hydroxytryptamine and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

serum(n=5)

92-105%

90-100%

87-105%

EDTA plasma(n=5)

82-100%

85-98%

84-93%

heparin plasma(n=5)

80-100%

85-100%

80-97%

CV(%)

Intra-Assay: CV<8%

Inter-Assay: CV<10%

Note

For Research Use Only

 

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