FineTest
SKU:ER1427
Rat VF(Visfatin) ELISA Kit
Rat VF(Visfatin) ELISA Kit
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Product Name
Rat VF(Visfatin) ELISA Kit
Alias
VF ELISA Kit, Visfatin ELISA Kit, NAMPT ELISA Kit, NAmPRTase ELISA Kit, NMPRTase ELISA Kit, PBEF1 ELISA Kit, Visfatin ELISA Kit, NAmPRTase ELISA Kit, Nampt ELISA Kit, nicotinamide phosphoribosyltransferase ELISA Kit, Pre-B cell-enhancing factor ELISA Kit, pre-B-cell colony enhancing factor 1 ELISA Kit, Pre-B-cell colony-enhancing factor 1 ELISA Kit, VISFATIN ELISA Kit, Visfatin ELISA Kit, PBEF ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
VF ELISA Kit allows for the in vitro quantitative determination of VF concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Rat
UniProt ID
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of VF and the recovery rates were calculated by comparing the measured value to the expected amount of VF in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
87-104 |
95 |
|
EDTA plasma(n=5) |
88-105 |
92 |
|
heparin plasma(n=5) |
88-102 |
97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-105% |
89-100% |
86-105% |
|
EDTA plasma(n=5) |
89-99% |
83-97% |
83-100% |
|
heparin plasma(n=5) |
80-96% |
80-88% |
80-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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