FineTest
SKU:ER1404
Rat TRF(Transferrin) ELISA Kit
Rat TRF(Transferrin) ELISA Kit
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Product Name
Rat TRF(Transferrin) ELISA Kit
Alias
TRF ELISA Kit, PRO1557 ELISA Kit, PRO2086 ELISA Kit, TFQTL1 ELISA Kit, Siderophilin ELISA Kit, Beta-1 metal-binding globulin ELISA Kit, Beta-1 metal-binding globulin ELISA Kit, DKFZp781D0156 ELISA Kit, EC 3.4.21 ELISA Kit, serotransferrin ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
TRF ELISA Kit allows for the in vitro quantitative determination of TRF concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
1.563-100ng/ml
Sensitivity
< 0.938ng/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of TRF and the recovery rates were calculated by comparing the measured value to the expected amount of TRF in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
89-101 |
95 |
|
EDTA plasma(n=5) |
89-100 |
96 |
|
heparin plasma(n=5) |
90-103 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TRF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
93-104% |
93-103% |
85-101% |
|
EDTA plasma(n=5) |
89-100% |
85-100% |
85-97% |
|
heparin plasma(n=5) |
80-98% |
86-99% |
81-93% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
