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SKU:ER1344
Rat Smad4(Mothers Against Decapentaplegic Homolog 4) ELISA Kit
Rat Smad4(Mothers Against Decapentaplegic Homolog 4) ELISA Kit
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Product Name
Rat Smad4(Mothers Against Decapentaplegic Homolog 4) ELISA Kit
Alias
Smad4 ELISA Kit, MADH4 ELISA Kit, MAD homolog 4 ELISA Kit, Mothers against DPP homolog 4 ELISA Kit, Deletion target in pancreatic carcinoma 4 ELISA Kit, SMAD family member 4 ELISA Kit, SMAD 4 ELISA Kit, Smad4 ELISA Kit, Hsmad4 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Smad4 ELISA Kit allows for the in vitro quantitative determination of Smad4 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 18.75pg/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Smad4 and the recovery rates were calculated by comparing the measured value to the expected amount of Smad4 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
89-102 |
95 |
|
EDTA plasma(n=5) |
87-103 |
96 |
|
heparin plasma(n=5) |
86-97 |
90 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Smad4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
89-98% |
89-103% |
87-101% |
|
EDTA plasma(n=5) |
92-100% |
85-101% |
88-101% |
|
heparin plasma(n=5) |
80-93% |
83-98% |
84-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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