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Rat Smad3(Mothers Against Decapentaplegic Homolog 3) ELISA Kit
Rat Smad3(Mothers Against Decapentaplegic Homolog 3) ELISA Kit
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Product Name
Rat Smad3(Mothers Against Decapentaplegic Homolog 3) ELISA Kit
Alias
Smad3 ELISA Kit, MADH3 ELISA Kit, SMAD family member 3 ELISA Kit, hMAD-3 ELISA Kit, hSMAD3 ELISA Kit, MAD homolog 3 ELISA Kit, mad protein homolog ELISA Kit, MAD ELISA Kit, mothers against decapentaplegic homolog 3 ELISA Kit, MAD ELISA Kit, mothers against decapentaplegic homolog 3 ELISA Kit, Drosophila ELISA Kit, mad3 ELISA Kit, Mad3 ELISA Kit, MADH3mad homolog JV15-2 ELISA Kit, mothers against decapentaplegic homolog 3 ELISA Kit, Mothers against DPP homolog 3 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Smad3 ELISA Kit allows for the in vitro quantitative determination of Smad3 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Smad3 and the recovery rates were calculated by comparing the measured value to the expected amount of Smad3 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
90-105 |
96 |
|
EDTA plasma(n=5) |
85-99 |
92 |
|
heparin plasma(n=5) |
87-105 |
97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Smad3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
91-103% |
92-104% |
85-94% |
|
EDTA plasma(n=5) |
85-101% |
87-96% |
84-101% |
|
heparin plasma(n=5) |
81-98% |
80-93% |
80-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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