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Rat sCD14(Soluble Cluster of Differentiation 14) ELISA Kit
Rat sCD14(Soluble Cluster of Differentiation 14) ELISA Kit
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Product Name
Rat sCD14(Soluble Cluster of Differentiation 14) ELISA Kit
Alias
sCD14 ELISA Kit, Soluble Cluster of Differentiation14 ELISA Kit, CD14 ELISA Kit, CD14 antigen ELISA Kit, CD14 molecule ELISA Kit, monocyte differentiation antigen CD14 ELISA Kit, Myeloid cell-specific leucine-rich glycoprotein ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
sCD14 ELISA Kit allows for the in vitro quantitative determination of sCD14 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
1.563-100ng/ml
Sensitivity
< 0.938ng/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of sCD14 and the recovery rates were calculated by comparing the measured value to the expected amount of sCD14 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
87-100 |
94 |
|
EDTA plasma(n=5) |
88-95 |
91 |
|
heparin plasma(n=5) |
88-102 |
96 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of sCD14 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
91-100% |
86-102% |
89-101% |
|
EDTA plasma(n=5) |
82-99% |
85-100% |
87-97% |
|
heparin plasma(n=5) |
80-92% |
83-99% |
81-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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