FineTest
SKU:ER1283
Rat PPAR-α(Peroxisome ProlifeRators Activator Receptors alpha) ELISA Kit
Rat PPAR-α(Peroxisome ProlifeRators Activator Receptors alpha) ELISA Kit
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Product Name
Rat PPAR-α(Peroxisome ProlifeRators Activator Receptors alpha) ELISA Kit
Alias
PPAR-α ELISA Kit, Peroxisome ProlifeRators Activator Receptors alpha ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
PPAR-α ELISA Kit allows for the in vitro quantitative determination of PPAR-α concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of PPAR-α and the recovery rates were calculated by comparing the measured value to the expected amount of PPAR-α in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
90-104 |
98 |
|
EDTA plasma(n=5) |
85-104 |
93 |
|
heparin plasma(n=5) |
89-103 |
97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PPAR-α and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
91-104% |
86-103% |
91-105% |
|
EDTA plasma(n=5) |
87-97% |
90-101% |
84-95% |
|
heparin plasma(n=5) |
80-92% |
80-100% |
80-87% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
