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SKU:ER1274
Rat PKCα(Protein Kinase Cα) ELISA Kit
Rat PKCα(Protein Kinase Cα) ELISA Kit
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Product Name
Rat PKCα(Protein Kinase Cα) ELISA Kit
Alias
PKCα ELISA Kit, PKCA ELISA Kit, PRKCA ELISA Kit, AAG6 ELISA Kit, aging-associated gene 6 ELISA Kit, MGC129901 ELISA Kit, PKC-A ELISA Kit, PKC-alpha ELISA Kit, PKCAMGC129900 ELISA Kit, PRKACA ELISA Kit, protein kinase C alpha type ELISA Kit, protein kinase C ELISA Kit, alpha ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
PKCα ELISA Kit allows for the in vitro quantitative determination of PKCα concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of PKCα and the recovery rates were calculated by comparing the measured value to the expected amount of PKCα in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
86-105 |
93 |
|
EDTA plasma(n=5) |
89-105 |
98 |
|
heparin plasma(n=5) |
85-105 |
97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PKCα and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
90-102% |
86-102% |
87-103% |
|
EDTA plasma(n=5) |
88-96% |
82-94% |
83-96% |
|
heparin plasma(n=5) |
82-97% |
80-100% |
82-96% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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