FineTest
SKU:ER1270
Rat PKCB1(Protein Kinase C Beta 1) ELISA Kit
Rat PKCB1(Protein Kinase C Beta 1) ELISA Kit
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Product Name
Rat PKCB1(Protein Kinase C Beta 1) ELISA Kit
Alias
PKCB1 ELISA Kit, PRKCB ELISA Kit, PRKCB1 ELISA Kit, PRKCB2 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
PKCB1 ELISA Kit allows for the in vitro quantitative determination of PKCB1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of PKCB1 and the recovery rates were calculated by comparing the measured value to the expected amount of PKCB1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
90-102 |
98 |
|
EDTA plasma(n=5) |
88-100 |
95 |
|
heparin plasma(n=5) |
86-103 |
97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PKCB1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-100% |
89-101% |
85-98% |
|
EDTA plasma(n=5) |
87-99% |
86-100% |
84-96% |
|
heparin plasma(n=5) |
93-95% |
83-92% |
80-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
