FineTest
SKU:ER1268
Rat PKB(Protein Kinase B) ELISA Kit
Rat PKB(Protein Kinase B) ELISA Kit
Couldn't load pickup availability
Product Name
Rat PKB(Protein Kinase B) ELISA Kit
Alias
Akt1 ELISA Kit, PKB alpha ELISA Kit, PRKBA ELISA Kit, RAC-alpha ELISA Kit, AKT ELISA Kit, PRKBA ELISA Kit, Protein kinase B ELISA Kit, PKB ELISA Kit, Proto-oncogene c-Akt ELISA Kit, rac protein kinase alpha ELISA Kit, RAC-ALPHA ELISA Kit, RAC-alpha serine ELISA Kit, threonine-protein kinase ELISA Kit, RAC-PK-alpha ELISA Kit, RACPKB-ALPHA ELISA Kit, v-akt murine thymoma viral oncogene homolog 1 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
PKB ELISA Kit allows for the in vitro quantitative determination of PKB concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of PKB and the recovery rates were calculated by comparing the measured value to the expected amount of PKB in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
88-105 |
95 |
|
EDTA plasma(n=5) |
86-97 |
93 |
|
heparin plasma(n=5) |
87-102 |
96 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PKB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
90-103% |
86-103% |
90-103% |
|
EDTA plasma(n=5) |
87-97% |
83-98% |
84-98% |
|
heparin plasma(n=5) |
81-98% |
81-99% |
86-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
Share
