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SKU:ER1268

Rat PKB(Protein Kinase B) ELISA Kit

Rat PKB(Protein Kinase B) ELISA Kit

Regular price $450.00 USD
Regular price Sale price $450.00 USD
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size
Sensitivity
Range

Product Name

Rat PKB(Protein Kinase B) ELISA Kit

Alias

Akt1 ELISA Kit, PKB alpha ELISA Kit, PRKBA ELISA Kit, RAC-alpha ELISA Kit, AKT ELISA Kit, PRKBA ELISA Kit, Protein kinase B ELISA Kit, PKB ELISA Kit, Proto-oncogene c-Akt ELISA Kit, rac protein kinase alpha ELISA Kit, RAC-ALPHA ELISA Kit, RAC-alpha serine ELISA Kit, threonine-protein kinase ELISA Kit, RAC-PK-alpha ELISA Kit, RACPKB-ALPHA ELISA Kit, v-akt murine thymoma viral oncogene homolog 1 ELISA Kit

Detection Method

Sandwich ELISA, Double Antibody

Application

PKB ELISA Kit allows for the in vitro quantitative determination of PKB concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Species

Rat

UniProt ID

P47196

Standard Curve

Storage

2-8 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of PKB and the recovery rates were calculated by comparing the measured value to the expected amount of PKB in samples.

Matrix

Recovery range(%)

Average(%)

serum(n=5)

88-105

95

EDTA plasma(n=5)

86-97

93

heparin plasma(n=5)

87-102

96

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PKB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

serum(n=5)

90-103%

86-103%

90-103%

EDTA plasma(n=5)

87-97%

83-98%

84-98%

heparin plasma(n=5)

81-98%

81-99%

86-100%

CV(%)

Intra-Assay: CV<8%

Inter-Assay: CV<10%

Note

For Research Use Only

 

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