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Rat PEPCK(Phosphoenolpyruvate Carboxykinase) ELISA Kit
Rat PEPCK(Phosphoenolpyruvate Carboxykinase) ELISA Kit
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Product Name
Rat PEPCK(Phosphoenolpyruvate Carboxykinase) ELISA Kit
Alias
PEPCK ELISA Kit, PEPCK1 ELISA Kit, PEPCK-C ELISA Kit, EC 4.1.1.32 ELISA Kit, MGC22652 ELISA Kit, PEPCK1 ELISA Kit, PEPCKC ELISA Kit, PEPCK-CPEP carboxykinase ELISA Kit, phosphoenolpyruvate carboxykinase 1 ELISA Kit, soluble ELISA Kit, phosphoenolpyruvate carboxykinase ELISA Kit, cytosolic ELISA Kit, phosphoenolpyruvate carboxykinase ELISA Kit, cytosolic [GTP] ELISA Kit, Phosphoenolpyruvate carboxylase ELISA Kit, phosphopyruvate carboxylase ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
PEPCK ELISA Kit allows for the in vitro quantitative determination of PEPCK concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of PEPCK and the recovery rates were calculated by comparing the measured value to the expected amount of PEPCK in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
86-103 |
93 |
|
EDTA plasma(n=5) |
87-105 |
99 |
|
heparin plasma(n=5) |
90-102 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PEPCK and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-102% |
86-100% |
85-105% |
|
EDTA plasma(n=5) |
85-97% |
83-101% |
88-100% |
|
heparin plasma(n=5) |
80-94% |
82-100% |
86-96% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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