FineTest
SKU:ER1241
Rat PDGFA(Platelet Derived Growth Factor Subunit A) ELISA Kit
Rat PDGFA(Platelet Derived Growth Factor Subunit A) ELISA Kit
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Product Name
Rat PDGFA(Platelet Derived Growth Factor Subunit A) ELISA Kit
Alias
PDGFA ELISA Kit, PDGF-A ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
PDGFA ELISA Kit allows for the in vitro quantitative determination of PDGFA concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of PDGFA and the recovery rates were calculated by comparing the measured value to the expected amount of PDGFA in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
87-97 |
93 |
|
EDTA plasma(n=5) |
86-103 |
95 |
|
heparin plasma(n=5) |
88-104 |
92 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PDGFA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
89-105% |
86-98% |
87-105% |
|
EDTA plasma(n=5) |
82-101% |
90-99% |
84-93% |
|
heparin plasma(n=5) |
89-100% |
80-95% |
82-93% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
