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Rat PACAP-27(Pituitary Adenylate Cyclase Activating Polypeptide 27) ELISA Kit
Rat PACAP-27(Pituitary Adenylate Cyclase Activating Polypeptide 27) ELISA Kit
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Product Name
Rat PACAP-27(Pituitary Adenylate Cyclase Activating Polypeptide 27) ELISA Kit
Alias
PACAP-27 ELISA Kit
Detection Method
Competitive ELISA, Coated with Antigen
Application
PACAP-27 ELISA Kit allows for the in vitro quantitative determination of PACAP-27 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
7.813-500pg/ml
Sensitivity
< 4.688pg/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of PACAP-27 and the recovery rates were calculated by comparing the measured value to the expected amount of PACAP-27 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
90-104 |
96 |
|
EDTA plasma(n=5) |
86-96 |
93 |
|
heparin plasma(n=5) |
91-100 |
97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PACAP-27 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
88-97% |
88-98% |
89-105% |
|
EDTA plasma(n=5) |
82-101% |
82-100% |
83-98% |
|
heparin plasma(n=5) |
83-95% |
82-97% |
82-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
