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Rat PⅢNT(Procollagen Ⅲ N-Terminal Peptide) ELISA Kit
Rat PⅢNT(Procollagen Ⅲ N-Terminal Peptide) ELISA Kit
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Product Name
Rat PIIINP(N-terminal propeptide of procollagen type III)ELISA Kit
Alias
PIIINP ELISA Kit, N-terminal propeptide of procollagen type III ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
PIIINPELISA Kit allows for the in vitro quantitative determination of PⅢNT concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
78.125-5000pg/ml
Sensitivity
< 46.875pg/ml
Species
Rat
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of PⅢNT and the recovery rates were calculated by comparing the measured value to the expected amount of PⅢNT in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
94-103 |
96 |
|
EDTA plasma(n=5) |
86-103 |
94 |
|
heparin plasma(n=5) |
89-103 |
97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PⅢNT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
88-100% |
86-101% |
86-102% |
|
EDTA plasma(n=5) |
87-101% |
84-98% |
83-100% |
|
heparin plasma(n=5) |
81-98% |
84-99% |
81-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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