FineTest
SKU:ER1214
Rat OPN(Osteopontin) ELISA Kit
Rat OPN(Osteopontin) ELISA Kit
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Product Name
Rat OPN(Osteopontin) ELISA Kit
Alias
OPN ELISA Kit, Osteopontin ELISA Kit, Eta-1 ELISA Kit, Spp1 ELISA Kit, BNSP ELISA Kit, Bone sialoprotein 1 ELISA Kit, MGC110940 ELISA Kit, Nephropontin ELISA Kit, secreted phosphoprotein-1 ELISA Kit, osteopontin ELISA Kit, bone sialoprotein ELISA Kit, SPP-1 ELISA Kit, SPP1 ELISA Kit, CALPHA1 fusion ELISA Kit, Urinary stone protein ELISA Kit, uropontin ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
OPN ELISA Kit allows for the in vitro quantitative determination of OPN concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
39.063-2500pg/ml
Sensitivity
< 23.438pg/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of OPN and the recovery rates were calculated by comparing the measured value to the expected amount of OPN in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
90-104 |
97 |
|
EDTA plasma(n=5) |
89-97 |
92 |
|
heparin plasma(n=5) |
88-105 |
97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of OPN and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-105% |
87-102% |
88-101% |
|
EDTA plasma(n=5) |
83-94% |
90-100% |
88-95% |
|
heparin plasma(n=5) |
87-93% |
82-100% |
83-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
