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SKU:ER1210
Rat OFQ/N(Orphanin FQ/Nociceptin) ELISA Kit
Rat OFQ/N(Orphanin FQ/Nociceptin) ELISA Kit
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Product Name
Rat OFQ/N(Orphanin FQ/Nociceptin) ELISA Kit
Alias
OFQ ELISA Kit, N ELISA Kit
Detection Method
Competitive ELISA, Coated with Antigen
Application
OFQ/N ELISA Kit allows for the in vitro quantitative determination of OFQ/N concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
1.563-100pg/ml
Sensitivity
< 0.938pg/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of OFQ/N and the recovery rates were calculated by comparing the measured value to the expected amount of OFQ/N in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
88-104 |
92 |
|
EDTA plasma(n=5) |
92-103 |
98 |
|
heparin plasma(n=5) |
86-97 |
92 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of OFQ/N and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-103% |
89-103% |
85-102% |
|
EDTA plasma(n=5) |
83-95% |
83-99% |
84-99% |
|
heparin plasma(n=5) |
84-97% |
80-99% |
81-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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