FineTest
SKU:ER1206
Rat OCLN(Occludin) ELISA Kit
Rat OCLN(Occludin) ELISA Kit
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Product Name
Rat OCLN(Occludin) ELISA Kit
Alias
OCLN ELISA Kit, BLCPMG ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
OCLN ELISA Kit allows for the in vitro quantitative determination of OCLN concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of OCLN and the recovery rates were calculated by comparing the measured value to the expected amount of OCLN in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
89-102 |
93 |
|
EDTA plasma(n=5) |
85-104 |
94 |
|
heparin plasma(n=5) |
87-105 |
97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of OCLN and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-99% |
92-103% |
85-103% |
|
EDTA plasma(n=5) |
83-101% |
85-94% |
82-89% |
|
heparin plasma(n=5) |
84-93% |
89-96% |
81-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
