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Rat NF-κB p65(Nuclear Factor-κB P65) ELISA Kit
Rat NF-κB p65(Nuclear Factor-κB P65) ELISA Kit
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Product Name
Rat NF-κB p65(Nuclear Factor-κB P65) ELISA Kit
Alias
NF-κB p65 ELISA Kit, NFKB3 ELISA Kit, p65RelA ELISA Kit, NFKB3v-rel avian reticuloendotheliosis viral oncogene homolog A ELISA Kit, nuclear factor ofkappa light polypeptide gene enhancer in B-cells 3 ELISA Kit, p65 ELISA Kit, Nuclear factor NF-kappa-B p65 subunit ELISA Kit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3transcription factor p65 ELISA Kit, p65 ELISA Kit, v-rel reticuloendotheliosis viral oncogene homolog A ELISA Kit, avian ELISA Kit, v-rel reticuloendotheliosis viral oncogene homolog A ELISA Kit, nuclear factor of kappalight polypeptide gene enhancer in B-cells 3 ELISA Kit, p65 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
NF-κB p65 ELISA Kit allows for the in vitro quantitative determination of NF-κB p65 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
78.125-5000pg/ml
Sensitivity
< 46.875pg/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of NF-κB p65 and the recovery rates were calculated by comparing the measured value to the expected amount of NF-κB p65 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
89-92 |
91 |
|
EDTA plasma(n=5) |
92-99 |
95 |
|
heparin plasma(n=5) |
92-103 |
99 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NF-κB p65 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-104% |
89-102% |
88-100% |
|
EDTA plasma(n=5) |
82-94% |
89-100% |
91-100% |
|
heparin plasma(n=5) |
87-100% |
88-97% |
87-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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