FineTest
SKU:ER1186
Rat NF-κB(Nuclear Factor Kappa B) ELISA Kit
Rat NF-κB(Nuclear Factor Kappa B) ELISA Kit
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Product Name
Rat NF-κB(Nuclear Factor Kappa B) ELISA Kit
Alias
NF-κB ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
NF-κB ELISA Kit allows for the in vitro quantitative determination of NF-κB concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Rat
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of NF-κB and the recovery rates were calculated by comparing the measured value to the expected amount of NF-κB in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
88-103 |
96 |
|
EDTA plasma(n=5) |
90-101 |
97 |
|
heparin plasma(n=5) |
91-102 |
96 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NF-κB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
87-104% |
86-99% |
95-103% |
|
EDTA plasma(n=5) |
86-96% |
83-98% |
83-99% |
|
heparin plasma(n=5) |
82-95% |
84-96% |
86-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
