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SKU:ER1183
Rat NEF3(Neurofilament 3) ELISA Kit
Rat NEF3(Neurofilament 3) ELISA Kit
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Product Name
Rat NEF3(Neurofilament 3) ELISA Kit
Alias
NEF3 ELISA Kit, NEFM ELISA Kit, NEF3neurofilament medium polypeptide ELISA Kit, neurofilament ELISA Kit, medium polypeptide ELISA Kit, neurofilament ELISA Kit, medium polypeptide 150kDa ELISA Kit, neurofilament-3 ELISA Kit, 150 kD medium ELISA Kit, NF-M160 kDa neurofilament protein ELISA Kit, NFMNeurofilament triplet M protein ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
NEF3 ELISA Kit allows for the in vitro quantitative determination of NEF3 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 18.75pg/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of NEF3 and the recovery rates were calculated by comparing the measured value to the expected amount of NEF3 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-97 |
92 |
|
EDTA plasma(n=5) |
87-94 |
91 |
|
heparin plasma(n=5) |
88-105 |
96 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NEF3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
90-105% |
89-103% |
87-97% |
|
EDTA plasma(n=5) |
83-95% |
82-99% |
82-96% |
|
heparin plasma(n=5) |
84-100% |
81-99% |
83-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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