FineTest
SKU:ER1098
Rat IL-27(Interleukin 27) ELISA Kit
Rat IL-27(Interleukin 27) ELISA Kit
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Product Name
Rat IL-27(Interleukin 27) ELISA Kit
Alias
IL-27 ELISA Kit, IL-27 subunit alpha ELISA Kit, IL-27-A ELISA Kit, IL27-A ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
IL-27 ELISA Kit allows for the in vitro quantitative determination of IL-27 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IL-27 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-27 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-104 |
94 |
|
EDTA plasma(n=5) |
92-104 |
99 |
|
heparin plasma(n=5) |
89-100 |
94 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-27 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-89% |
93-104% |
87-105% |
|
EDTA plasma(n=5) |
84-101% |
91-101% |
84-101% |
|
heparin plasma(n=5) |
81-93% |
81-99% |
81-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
