FineTest
SKU:ER1099
Rat IL-2Rα(Interleukin-2 Receptor-α) ELISA Kit
Rat IL-2Rα(Interleukin-2 Receptor-α) ELISA Kit
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Product Name
Rat IL-2Rα(Interleukin-2 Receptor-α) ELISA Kit
Alias
IL-2Rα ELISA Kit, CD25 ELISA Kit, Interleukin 2 Receptor Alpha ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
IL-2Rα ELISA Kit allows for the in vitro quantitative determination of IL-2Rα concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IL-2Rα and the recovery rates were calculated by comparing the measured value to the expected amount of IL-2Rα in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
88-99 |
94 |
|
EDTA plasma(n=5) |
91-101 |
97 |
|
heparin plasma(n=5) |
90-102 |
96 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-2Rα and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-104% |
85-98% |
87-102% |
|
EDTA plasma(n=5) |
89-101% |
85-100% |
92-101% |
|
heparin plasma(n=5) |
88-99% |
83-100% |
80-93% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
