FineTest
SKU:ER1086
Rat IL-12(Interleukin 12) ELISA Kit
Rat IL-12(Interleukin 12) ELISA Kit
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Product Name
Rat IL-12(Interleukin 12) ELISA Kit
Alias
IL-12 ELISA Kit, NKSF ELISA Kit, CLMF ELISA Kit, Interleukin 12 ELISA Kit, Natural Killer Cell Stimulatory Factor ELISA Kit, Cytotoxic Lymphocyte MatuRation Factor ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
IL-12 ELISA Kit allows for the in vitro quantitative determination of IL-12 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Rat
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IL-12 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-12 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-105 |
93 |
|
EDTA plasma(n=5) |
87-100 |
94 |
|
heparin plasma(n=5) |
88-105 |
94 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-12 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-97% |
87-105% |
85-97% |
|
EDTA plasma(n=5) |
82-101% |
94-101% |
85-96% |
|
heparin plasma(n=5) |
83-97% |
82-97% |
81-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
