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SKU:ER1084
Rat IKBα(Inhibitory Subunit of NF-κBα) ELISA Kit
Rat IKBα(Inhibitory Subunit of NF-κBα) ELISA Kit
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Product Name
Rat IKBα(Inhibitory Subunit of NF-κBα) ELISA Kit
Alias
IKBα ELISA Kit, MAD-3 ELISA Kit, NFKBIA ELISA Kit, alpha ELISA Kit, I-kappa-B-alpha ELISA Kit, IkappaBalphaNF-kappa-B inhibitor alpha ELISA Kit, IKBAikB-alpha ELISA Kit, IkB-alpha ELISA Kit, MAD3 ELISA Kit, MAD-3 ELISA Kit, Major histocompatibility complex enhancer-binding protein MAD3 ELISA Kit, NFKBI ELISA Kit, NFKBIA ELISA Kit, nuclear factor of kappa light chain gene enhancer in B-cells ELISA Kit, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
IKBα ELISA Kit allows for the in vitro quantitative determination of IKBα concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IKBα and the recovery rates were calculated by comparing the measured value to the expected amount of IKBα in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
88-98 |
94 |
|
EDTA plasma(n=5) |
88-105 |
96 |
|
heparin plasma(n=5) |
85-104 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IKBα and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
92-104% |
85-95% |
87-92% |
|
EDTA plasma(n=5) |
85-101% |
83-99% |
93-100% |
|
heparin plasma(n=5) |
83-100% |
83-89% |
81-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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