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SKU:ER1084

Rat IKBα(Inhibitory Subunit of NF-κBα) ELISA Kit

Rat IKBα(Inhibitory Subunit of NF-κBα) ELISA Kit

Regular price $450.00 USD
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size
Sensitivity
Range

Product Name

Rat IKBα(Inhibitory Subunit of NF-κBα) ELISA Kit

Alias

IKBα ELISA Kit, MAD-3 ELISA Kit, NFKBIA ELISA Kit, alpha ELISA Kit, I-kappa-B-alpha ELISA Kit, IkappaBalphaNF-kappa-B inhibitor alpha ELISA Kit, IKBAikB-alpha ELISA Kit, IkB-alpha ELISA Kit, MAD3 ELISA Kit, MAD-3 ELISA Kit, Major histocompatibility complex enhancer-binding protein MAD3 ELISA Kit, NFKBI ELISA Kit, NFKBIA ELISA Kit, nuclear factor of kappa light chain gene enhancer in B-cells ELISA Kit, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor ELISA Kit

Detection Method

Sandwich ELISA, Double Antibody

Application

IKBα ELISA Kit allows for the in vitro quantitative determination of IKBα concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.313-20ng/ml

Sensitivity

< 0.188ng/ml

Species

Rat

UniProt ID

Q63746

Standard Curve

Storage

2-8 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of IKBα and the recovery rates were calculated by comparing the measured value to the expected amount of IKBα in samples.

Matrix

Recovery range(%)

Average(%)

serum(n=5)

88-98

94

EDTA plasma(n=5)

88-105

96

heparin plasma(n=5)

85-104

95

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IKBα and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

serum(n=5)

92-104%

85-95%

87-92%

EDTA plasma(n=5)

85-101%

83-99%

93-100%

heparin plasma(n=5)

83-100%

83-89%

81-99%

CV(%)

Intra-Assay: CV<8%

Inter-Assay: CV<10%

Note

For Research Use Only

 

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