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SKU:ER1057
Rat HSP-70(Heat Shock Protein 70) ELISA Kit
Rat HSP-70(Heat Shock Protein 70) ELISA Kit
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Product Name
Rat HSP-70(Heat Shock Protein 70) ELISA Kit
Alias
DAAP-21F2.7 ELISA Kit, FLJ54328 ELISA Kit, HSP70-1B ELISA Kit, HSP70-2 ELISA Kit, HSPA1A ELISA Kit, heat shock 70kD protein 1B ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
HSP-70 ELISA Kit allows for the in vitro quantitative determination of HSP-70 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of HSP-70 and the recovery rates were calculated by comparing the measured value to the expected amount of HSP-70 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-105 |
95 |
|
EDTA plasma(n=5) |
91-100 |
96 |
|
heparin plasma(n=5) |
86-101 |
93 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HSP-70 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-102% |
89-98% |
86-97% |
|
EDTA plasma(n=5) |
83-99% |
82-98% |
85-99% |
|
heparin plasma(n=5) |
81-99% |
86-98% |
92-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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