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Rat HMG-CoA(3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase) ELISA Kit
Rat HMG-CoA(3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase) ELISA Kit
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Product Name
Rat HMG-CoA(3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase) ELISA Kit
Alias
HMG-CoA ELISA Kit, Hmgcr ELISA Kit, 3-hydroxy-3-methylglutaryl-coenzyme A reductase ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
HMG-CoA ELISA Kit allows for the in vitro quantitative determination of HMG-CoA concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of HMG-CoA and the recovery rates were calculated by comparing the measured value to the expected amount of HMG-CoA in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
90-99 |
95 |
|
EDTA plasma(n=5) |
86-105 |
98 |
|
heparin plasma(n=5) |
87-102 |
93 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HMG-CoA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-103% |
86-103% |
87-104% |
|
EDTA plasma(n=5) |
84-98% |
83-97% |
87-99% |
|
heparin plasma(n=5) |
91-99% |
85-98% |
81-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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