FineTest
SKU:ER1038
Rat HK(Hexokinase) ELISA Kit
Rat HK(Hexokinase) ELISA Kit
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Product Name
Rat HK(Hexokinase) ELISA Kit
Alias
HK ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
HK ELISA Kit allows for the in vitro quantitative determination of HK concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 18.75pg/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of HK and the recovery rates were calculated by comparing the measured value to the expected amount of HK in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
86-96 |
92 |
|
EDTA plasma(n=5) |
88-105 |
98 |
|
heparin plasma(n=5) |
88-102 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HK and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
89-103% |
97-105% |
88-98% |
|
EDTA plasma(n=5) |
86-99% |
83-101% |
85-101% |
|
heparin plasma(n=5) |
85-99% |
80-93% |
81-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
