FineTest
SKU:ER1035
Rat HDL(High Density Lipoprotein) ELISA Kit
Rat HDL(High Density Lipoprotein) ELISA Kit
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Product Name
Rat HDL(High Density Lipoprotein) ELISA Kit
Alias
HDL ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
HDL ELISA Kit allows for the in vitro quantitative determination of HDL concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
3.125-200ng/ml
Sensitivity
< 1.875ng/ml
Species
Rat
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of HDL and the recovery rates were calculated by comparing the measured value to the expected amount of HDL in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
90-104 |
97 |
|
EDTA plasma(n=5) |
85-103 |
91 |
|
heparin plasma(n=5) |
88-105 |
96 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HDL and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-96% |
85-105% |
93-94% |
|
EDTA plasma(n=5) |
83-101% |
83-101% |
85-99% |
|
heparin plasma(n=5) |
83-94% |
80-99% |
81-93% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
