FineTest
SKU:ER0977
Rat GAP(Ras Gtpase Activating Protein) ELISA Kit
Rat GAP(Ras Gtpase Activating Protein) ELISA Kit
Couldn't load pickup availability
Product Name
Rat GAP(Ras Gtpase Activating Protein) ELISA Kit
Alias
GAP ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
GAP ELISA Kit allows for the in vitro quantitative determination of GAP concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of GAP and the recovery rates were calculated by comparing the measured value to the expected amount of GAP in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
89-105 |
95 |
|
EDTA plasma(n=5) |
86-105 |
95 |
|
heparin plasma(n=5) |
90-98 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GAP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-97% |
90-105% |
86-101% |
|
EDTA plasma(n=5) |
82-101% |
82-99% |
88-101% |
|
heparin plasma(n=5) |
89-100% |
82-97% |
80-91% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
