FineTest
SKU:ER0962
Rat fT4(Free Thyroxine) ELISA Kit
Rat fT4(Free Thyroxine) ELISA Kit
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Product Name
Rat fT4(Free Thyroxine) ELISA Kit
Alias
fT4 ELISA Kit, Free Thyroxine ELISA Kit, T4 ELISA Kit, Thyroxine ELISA Kit, Thyroxine ELISA Kit, total T4 ELISA Kit, total T4 ELISA Kit
Detection Method
Competitive ELISA, Coated with Antigen
Application
fT4 ELISA Kit allows for the in vitro quantitative determination of fT4 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
1.563-100pg/ml
Sensitivity
< 0.938pg/ml
Species
Rat
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of fT4 and the recovery rates were calculated by comparing the measured value to the expected amount of fT4 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
92-104 |
99 |
|
EDTA plasma(n=5) |
88-102 |
93 |
|
heparin plasma(n=5) |
87-104 |
96 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of fT4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-105% |
85-105% |
93-105% |
|
EDTA plasma(n=5) |
83-90% |
82-100% |
82-99% |
|
heparin plasma(n=5) |
84-91% |
83-99% |
81-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
