FineTest
SKU:ER0949
Rat FGF19(Fibroblast Growth Factor 19) ELISA Kit
Rat FGF19(Fibroblast Growth Factor 19) ELISA Kit
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Product Name
Rat FGF19(Fibroblast Growth Factor 19) ELISA Kit
Alias
FGF19 ELISA Kit, FGF-19 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
FGF19 ELISA Kit allows for the in vitro quantitative determination of FGF19 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of FGF19 and the recovery rates were calculated by comparing the measured value to the expected amount of FGF19 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-104 |
93 |
|
EDTA plasma(n=5) |
85-104 |
94 |
|
heparin plasma(n=5) |
88-105 |
96 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FGF19 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
94-98% |
86-102% |
88-103% |
|
EDTA plasma(n=5) |
82-101% |
84-95% |
85-95% |
|
heparin plasma(n=5) |
83-100% |
80-94% |
82-95% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
